Prokhorova E.E.1, Serebryakova M.S.2, Tokmakova A.S.1, Kudryavtsev I.V.2, Usmanova R.R.1, Ataev G.L.1 2018. [The analysis of cell composition in the hemolymph of three Planorbidae species (Gastropoda: Pulmonata)] // Invertebrate Zoology. Vol.15. No.1: 103–113 [in Russian, with English summary].
1 Herzen State Pedagogical University of Russia,Saint-Petersburg 191186 Russia. E-mail: firstname.lastname@example.org
2 Institute of Experimental Medicine RAMS, Saint-Petersburg 197376 Russia.
ABSTRACT: Cell compositon of the hemolymph of three Planonbidae snails (Biomphalaria glabrata, Planorbarius corneus and Planorbis planorbis) was examined. The flow-cytometric and the morphometric analysis revealed two main hemocyte types: a population of hyalinocytes and polymorphic fraction of granulocytes including two populations of large and small cells. Staining with specific fluorochromes SYTO62 (stain nucleic acids) and LysoTracker (accumulated in lysosomes) allowed to distinguish four functional subpopulations of hemocytes. The significant differences in the lysosomes concentration and accumulation of nucleic dye were revealed between cells belonging to different morphological populations. Hyalinocytes contain fewer lysosomes than granulocytes. Large granulocytes are characterized by the highest concentration of lysosomes and metabolic activity, and small granulocytes are varying in the degree of granularity and composition of the granules.
KEY WORDS: Biomphalaria glabrata, Planorbarius corneus, Planorbis planorbis, hemocytes, flow cytometry.