Silivanova E.A., Levchenko M.A., Shumilova P.A., Plashkina V.A. 2020. [Phosphatase and acetylcholinesterase activities in different stages of the life cycle of the housefly Musca domestica L.] // Euroasian Entomological Journal. Vol.19. No.3: 124–130 [in Russian, with English summary].
All-Russian Scientific Research Institute of Veterinary Entomology and Arachnology — Branch of Federal State Institution Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences (ASRIVEA — Branch of Tyumen Scientific Centre SB RAS), Institutskaya Str. 2, Tyumen 625041 Russia. E-mail:vniivea@mail.ru
ABSTRACT. Activities of detoxification enzymes are critical for the development of the metabolic resistance to insecticides in insects and the adaptation to insecticides at different stages of the insect development. The present study was aimed to assess the ontogenetic profile of activities of acidic and alkaline phosphatases and acetylcholinesterase in the housefly Musca domestica L. The enzyme activities were also evaluated in adult females and males of M. domestica. Biochemical studies showed that the activities of alkaline phosphatase and acetylcholinesterase significantly changed during the development of M. domestica from the egg stage to the adult insect, and these activities differed for male and female in adult insects. The most intense changes in the activity of these enzymes occurred at the pre-imaginal developmental stages. The alkaline phosphatase activity was minimal at the egg stage (1.72 ± 0.24 mmol/min/mg of protein) and maximum at the larval stage (20.83 ± 1.61 mmol/min/mg of protein). The acetylcholinesterase activity had also the lowest value in the eggs (0.75 ± 0.02 µmol/min/mg of protein), and reached a maximal value at the late pupa (12.92 ± 1.00 µmol/min/mg of protein). The acetylcholinesterase activities in 5 and 10 day-old males were 1.4 and 2 times higher than that of females, respectively. The alkaline phosphatase activities in 5 and 10 day-old males were 2 times lower than that of females. An important consequence of the observed differences of the alkaline phosphatase and acetylcholinesterase activities may be the different susceptibility to insecticides in individuals at different development stages, as well as in males and females of M. domestica.
KEY WORDS: acide phosphatase, alkaline phosphatase, acetylcholinesterase, detoxifying enzymes, egg, larva, pupa, adult insect, insect development stages.