Arapova M.Yu.1, Oyun N.Yu.2 2024. Molecular identification of Zeugodacus cucurbitae (Coquillett, 1899) (Diptera: Tephritidae) using PCR-RFLP // Euroasian Entomological Journal. Vol.23. No.6: 316–320 [in English].
1 Entomology Department, Faculty of Biology, Lomonosov Moscow State University, Leninskie Gory 1–12, Moscow 119234 Russia. E-mail: arapovamy@mail.ru https://orcid.org/0000-0002-7368-4971; FSBI All-Russian Plant Quarantine Center (FGBU “VNIIKR”), Pogranichnaya Str. 32, Moskovskaya Oblast, Bykovo 140150 Russia.
2 FSBI Centre for Strategic Planning and Management of Biomedical Health Risks of the Federal medical and biological agency, Pogodinskaya Str. 10–1, Moscow 119121 Russia. E-mail: nad_oyun@mail.ru https://orcid.org/0000-0001-9279-4386; Martsinovsky Institute of Medical Parasitology, Tropical and Vector-Borne Diseases, Sechenov University, Malaya Pirogovskaya Str. 20, Moscow 119435 Russia; Entomology Department, Faculty of Biology, Lomonosov Moscow State University, Leninskie Gory 1–12, Moscow 119234 Russia.
doi 10.15298/euroasentj.23.06.03
ABSTRACT. This paper discusses the method of molecular identification of the melon fly Zeugodacus cucurbitae (Coquillett) based on restriction fragment length polymorphism-polymerase chain reaction (PCR-RFLP). This approach, which involves the analysis of nucleotide composition of the 5’-end of the mitochondrial COI gene fragment, allows for the detection of differences in the nucleotide sequences of this region and serves as a rapid method for identification and differentiation Z. cucurbitae from other fruit fly species, which larvae can also be found in the fruits of cucurbit plants.
KEY WORDS: Fruit fly, molecular identification, COI gene, plant quarantine.