Lovtsova J.A.1, Kovalenko M.G.1, Gura N.A.1, Shipulin A.V.1, Kochiev M.V.2 2023. A new method of staining larvae of Lepidoptera using carbol fuchsin // Russian Entomol. J. Vol.32. No.2: 194–197 [in English].

1 All-Russian Plant Quarantine Center, Moscow Oblast, Bykovo 140150, Russia. E-mails: julialov@inbox.ru , bush_zbs@mail.ru , naguralex@mail.ru , schipulin.andrey2016@yandex.ru

2 Lebedev Physical Institute of the Russian Academy of Sciences, Moscow, Russia, 119991. E-mail: kochievmv@mail.ru

doi: 10.15298/rusentj.32.2.09

ABSTRACT. A new method of staining caterpillars using carbol fuchsin has been proposed. The recommended time of dipping larvae into carbol fuchsin is more than 2 minutes. Studying of stained specimens was carried out in glass containers filled with eucalyptus oil. Examination of stained specimens in alcohol is difficult because of rapid washout of carbol fuchsin. Further storage of larvae was carried out in alcohol due to the fact that eucalyptus oil damages the covers during prolonged storing.

KEY WORDS: Phycitini, Plodia interpunctella, caterpillars, staining, carbol fuchsin, moths, larvae, Lepidoptera.

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